Solutions/media used:

Ad-DF+++	Final Concentration	Volume (mL)
Ad. DMEM/F12	not applicable	485
GlutaMAX Supplement (200 mM)	2 mM	5
HEPES (1 M)	10 mM	5
Pen/strep (10,000 units/mL)	100 units/mL	5
Total		500

Once prepared, stored at 4°C for 4 weeks