| Digestion Buffer for Tumor Tissue | Final Concentration | Volume (μL) |
|---|---|---|
| Ad-DF+++ | not applicable | 3,745 |
| Collagenase type II (20 mg/mL) | 5 mg/mL | 1,250 |
| ROCK inhibitor (Y-27632) (10 mM) | 10 μM | 5 |
| Total | 5,000 |
Once prepared, stored at 4°C for 1 week
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