Surgical Preparation for Orthotopic Implantation
In a disinfected biological safety cabinet, prepare the surgical instruments as follows: (A) Tubes for Matrigel and HCC cell suspension on ice and 0.3-mL 31-gauge needle insulin syringes, (B) gauzes with 10% povidone-iodine solution, (C) gauzes with 70% ethanol, (D) sterilized surgical instruments, (E) autoclip applicator, (F) suture, (G) bead sterilizer, (H) ophthalmic ointment. Missing in the picture but required: an isoflurane chamber and nose cone, and a surgical microscope.