Extended Data Fig. 7. The ITCH and SIAH2 E3 ubiquitin ligases do not mediate heat shock-induced LATS degradation.
(a) Knockdown of ITCH and SIAH2 does not block the heat shock-induced LATS1 degradation. Immunoblotting has been performed two times with similar results.
(b) Verification of ITCH&SIAH2 siRNA knockdown efficiency by RT-PCR. Data are presented as mean ± s.d.; n = 3 biologically independent samples. Two-way ANOVA test. Source data are available online.