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. Author manuscript; available in PMC: 2021 May 16.
Published in final edited form as: Nat Cell Biol. 2020 Nov 16;22(12):1447–1459. doi: 10.1038/s41556-020-00602-9

Extended Data Fig. 1. Heat shock activates YAP.

Extended Data Fig. 1

(a) Heat shock induces YAP dephosphorylation in multiple cell lines: human colon cancer HCT116; human uveal melanoma MEL270; mouse fibroblast NIH3T3; mouse squamous cell carcinoma SCC7; mouse melanoma B16-OVA. Various tumor and non-tumor cell lines were seeded onto six well plates to achieve high density (100% confluence). After 24 h, cells were heat shocked at 43°C for the indicated times and collected for YAP phos-tag gel analyses.

(b) Heat shock induced a sustained YAP dephosphorylation.

(c) Mild heat shock-induced YAP dephosphorylation is cell type-dependent. High density HEK293A and A549 cells were heat shocked at 40°C for the indicated times and collected for YAP phos-tag gel analyses.

(d) Cold stress does not affect YAP phosphorylation. High density HEK293A cells were placed at 25°C or 4°C for 30 min and 1 h; then samples were collected for YAP phos-tag gel analyses. Immunoblotting in panels a-d has been performed two times with similar results. Source data are available online.