Table 1.

Selected TCR sequencing methods.

Technique	Single chain	Paired	Expensive	Quantitative	Maximum #of cells	Comments
Targeted V region PCR	X				>1,000,000	Efficiency dependent on primer design
Flow cytometry	X	X		X	>1,000,000	Limited # of TRAV & TRBV human antibodies
Adaptive Biotechnologies	X			X	>1,000,000
iRepertoire Immune Repertoire	X			X	>1,000,000
iRepertoire iPair	X	X	X	X	l00s	Can be paired with T cell phenotyping
scPCR	X	X	X	X	l00s	Can be paired with T cell phenotyping
ePCR	X	X	X	?	>1,000,000	May not be directly quantitative
Fluidigm Cl	X	X	X	X	800	Can be paired with scRNA-seq
10X Chromium	X	X	X	X	10,000	Can be paired with scRNA-seq