IMAC pH 8.8 Buffer (For Column Equilibrating and Washing)^a

Reagent	Final Concentration (mM)	Amount (mL)
Tris buffer pH 8.8 (1 M)	20	0.4
NaCl (5 M)	50	2
Imidazole (1 M)	30	0.6
Pyrogen-free H2O	n/a	17
Total	n/a	20