Figure 4.

A frequently observed outcome: number of hepatocytes isolated from different Percoll fractions, and the corresponding lipid content as measured by oil red O staining

(A) Viable hepatocyte populations from a representative isolation. The cell numbers from each Percoll gradient were measured using trypan blue and a manual hemocytometer. Values are expressed as a percentage of viable cells.

(B) Hepatocyte cell numbers from a representative isolation. The cell numbers and viability from each Percoll gradient was measured using trypan blue and a manual hemocytometer.

(C) Lipid staining using oil red O in hepatocytes from 90% and 25% Percoll gradients at 100 × g or 1,000 × g. Microscope images show results from a representative isolation using a 60× objective. Mayer’s hematoxylin solution is used as counterstain. Scale bar denotes 100 μm in the original image and 35 μm in the magnified image.