Digestion buffer

Reagent	Stock concentration	Final concentration	Amount
DMEM high glucose	1×	1×	89 mL
Penicillin/Streptomycin	100×	1% (v/v)	1 mL
Collagenase IV	n/a	1 mg/mL	100 mg
HEPES	10 g/L	10 μg/mL	100 μL
FBS	n/a	10% (v/v)	10 mL
Total			100 mL