Figure 8.

Representative Live-Cell Microscopy of DC:T Cell Interactions at Steady State in Collagen

(A) Syngeneic DCs (green) and activated T cells (red) were co-embedded into collagen gels and visualized by live-cell microscopy. Scale bar, 20 μm.

(B) DC and T cell tracks over the 30-min recording are shown.

(C) Mean 3D track velocities of DCs (green) and T cells (red). Black lines depict median values.

(D) DC:T cell contact duration of control or in the presence of Staphylococcus enterotoxin B (SEB). Black lines depict median values.