Reagents for Preparing Collagen Gels for IHC

Reagent	Amount	Storage condition
4% paraformaldehyde/5% sucrose solution	2 mL	20°C–22°C
Glycine buffer (0.15 M, pH 7)	2 mL	20°C–22°C
0.5% Triton X solution	2 mL	20°C–22°C
1% bovine serum albumin solution	2 mL	4°C
PBS + 0.2% Tween-20 wash solution	100 mL	20°C–22°C