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. 2020 Dec 8;1(3):100203. doi: 10.1016/j.xpro.2020.100203

Bovine collagen gel (1.7 mg/mL)

Reagent Final Concentration (mM or μM) Amount
10× MEM 0.74× 20 μL
7.5% sodium bicarbonate 0.28% 10 μL
3.1 mg/mL Bovine PureColl 1.7 mg/mL 148 μL
Cells in complete RPMI 92 μL
Total n/a 270 μL