REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human CD3 (clone: HIT3a)	Biolegend	Cat #300318
Human CD4 (clone: RPA-T4)	Biolegend	Cat #300537
Human CD11c (clone: 3.9)	Biolegend	Cat #337218
Human CD14 (clone: 63D3)	Biolegend	Cat #367116
Human CD45RO (clone: UCHL1)	Biolegend	Cat #304230
Human CD62L (clone: DREG-56)	Biolegend	Cat #304814
Human CD80 (clone: 2D10)	Biolegend	Cat #305218
Human CD86 (clone: IT2.2)	Biolegend	Cat #305406
Human CCR7 (clone: G043H7)	Biolegend	Cat# 353214
Human HLA-DR (clone: L243)	Biolegend	Cat #307628
anti-human CD3ε/CD28 antibody-coated Dynabeads	Life Technologies	Cat #11131D
Biological Samples
Buffy coats from healthy anonymized donors	Canadian Blood Services (CBS)	n/a
Chemicals, Peptides, and Recombinant Proteins
Human recombinant IL-2	Peprotech	Cat #200-02
Human recombinant IL-4	Biolegend	Cat #574008
Human recombinant GM-CSF	Biolegend	Cat #578606
Ficoll-Hypaque solution	GE Healthcare	Cat #17-1440-03
LPS from Salmonella Minnesota	Invivo Gen	Cat #tlrl-smlps
Fetal calf serum (FCS)	VWR Seradigm	Cat #1500-500
Nunclon Sphera flasks	Thermo Scientific	Cat #174951
GlutaMAX Supplement	Gibco	Cat #35050061
Sodium pyruvate (100 mM)	Gibco	Cat #11360070
HEPES (1 M)	Sigma	Cat #H3375-500G
Penicillin/streptomycin solution (10,000 U/mL)	Gibco	Cat #15140122
Tween 20	Sigma	Cat #P9416-100ML
Triton X-100	Sigma	Cat #T8787-50ML
Glycine	Sigma	Cat #G7126-100G
Bovine serum albumin	Sigma	Cat #A8806-5G
10× MEM	Gibco	Cat #11430-030
7.5% sodium bicarbonate solution	Gibco	Cat #25080-094
PureColl bovine collagen solution (3.1 mg/mL)	Advanced Biomatrix	Cat #5005-100ML
Glass microscope slides	Thermo Fisher	Cat #12-550-015
Glass coverslips (18 mm × 18 mm)	VWR	Cat #16004-094
Adhesive silicone isolators (1 mm thick)	EMS	Cat #70336-72
High vacuum grease	Dow Corning	Cat #Z273554
Critical Commercial Assays
Human CD14+ monocyte isolation kit	STEMCELL Technologies	Cat #17858
Human CD4+ T cell isolation kit	STEMCELL Technologies	Cat #17952
EasySep Buffer	STEMCELL Technologies	Cat #20144
Software and Algorithms
Imaris 8.2	Bitplane	n/a
ImarisFileConverter	Bitplane	n/a
ImageJ/Fiji	NIH/freeware	n/a
Prism 8	GraphPad	n/a
FlowJo	Treestar	n/a
MATLAB	MathWorks	n/a
Ultima Multiphoton Microscope	Bruker	n/a