Reagent	Final Concentration	Amount
Arg-tRNAArg or Arg-tRFArg (40.70 μM)	5 μM	6.25 μL
Angiotensin II (DRVYIHPF) (300 μM)	15 μM	2.5 μL
ATE1 (1,320 μg/mL; 22 μM)	3 μM	6.81 μL
10× buffer (500 mM HEPES, 250 mM KCl, 150 mM MgCl2, 1 mM DTT, pH 7.5)	1×	5 μL
DEPC-treated water	n/a	29.44 μL