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. 2020 Dec 16;1(3):100215. doi: 10.1016/j.xpro.2020.100215

Figure 2.

Figure 2

Breeding strategy for experimental mice

(A) Breeding strategy A illustrates the generation of experimental MADM mice for sorting of cells originating from a genetically defined lineage. Experimental mice contain red (TdT+) cells with unimaternal disomy (matUPD: 2x maternally expressed genes, 0x paternally expressed genes). Green (GFP+) cells carry unipaternal disomy (patUPD: 2x paternally expressed genes, 0x maternally expressed genes).

(B) Breeding strategy B illustrates reversal of fluorescent colors in cells with MADM-induced UPD. In this setup green (GFP+) cells contain matUPD and red (TdT+) cells carry patUPD.

(C) Breeding strategy C shows the generation of experimental MADM-LacZ mice for astrocyte sorting. Astrocytes are identified based on LacZ expression under the control of the human GFAP promoter. GFP+, LacZ+ astrocytes harbor matUPD, while TdT+, LacZ+ positive cells carry patUPD.

(D) Breeding strategy D indicates reversal of fluorescent colors in cells with MADM-induced UPD in experimental animals for MADM+LacZ astrocyte sorting. TdT+, LacZ+ cells carry matUPD and GFP+, LacZ+ cells harbor patUPD.

Parts of the figure and its legend are reused but adapted from previous publications (Hippenmeyer et al., 2013, Hippenmeyer et al., 2010, Laukoter et al., 2020b) with permission.