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Gating strategy for FACS experiments
(A) Gating steps for sorting MADM-labeled cells.
(B) Gating for red LacZ− population is set up based on a negative control (no XGFAP-LacZ expression).
(C) Gating for red astrocytes (TdT+, LacZ+).
(D) Gating for green LacZ− population is set up based on a negative control (no XGFAP-LacZ expression).
(E) Gating for green astrocytes (GFP+, LacZ+).
