Volumes of reagents are adjusted according to the amount of used tissue/sample

Tissue	Papain-DNase solution	Solution 2 (step 5)	Solution 2 (step 7)	DMEM/F12
Single cortex (embryonic brain)	650 μL	500 μL	250 μL	1,000 μL
One hemisphere (P0 animal)	650 μL	500 μL	250 μL	1,000 μL
Multiple embryonic cortices (up to 3 cortices)	1,300 μL	1,200 μL	300 μL	1,000 μL
Cortex of single individual (P0, adult animals)	1,300 μL	1,200 μL	300 μL	1,000 μL
Multiple cortices (up to 3 cortices at any postnatal age)	2,600 μL	2,400 μL	500 μL	2,000 μL