REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Papain vial source	Worthington	Cat#PAP2
DNase vial source	Worthington	Cat#D2
Inhibitor vial source	Worthington	Cat#OI-BSA
EBSS	Thermo Fisher Scientific	Cat#24010043
DMEM/F12	Thermo Fisher Scientific	Cat#21041025
Heat-inactivated fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat#10082147
Heat-inactivated horse serum (HS)	Thermo Fisher Scientific	Cat#26050088
Triton X 100	Sigma-Aldrich	Cat#T878
RNase inhibitor	Takara	Cat#2313A
RNase away	Thermo Fisher Scientific	Cat#10328011
RNase-free water	Thermo Fisher Scientific	Cat#10977035
Critical commercial assays
FACS Blue LacZ beta galactosidase detection kit	Abcam	Cat#ab189815
Experimental models: organisms/strains
Mouse: MADM-7-GT	The Jackson Laboratory	RRID:IMSR_JAX:021457
Mouse: MADM-7-TG	The Jackson Laboratory	RRID:IMSR_JAX:021458
Mouse: Emx1-Cre	The Jackson Laboratory	RRID:IMSR_JAX:005628
Mouse: XGFAP-LacZ	The Jackson Laboratory	RRID: IMSR JAX: 003487
Software and algorithms
FACS Diva	BD Biosciences	https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software
R statistical environment		https://www.r-project.org/
DESeq2	Love et al., 2014	http://www.bioconductor.org/
Seurat	Butler et al., 2018	http://www.bioconductor.org/
Other
FACS Aria III	BD Biosciences	N/A
AluminaSeal	Merck	Cat#Z740251-100EA
Hard-Shell PCR Plates (96-well)	Bio Rad	Cat#HSP9631
PCR tubes and caps, RNase-free, 0.2 mL (8-strip format)	Thermo Fisher Scientific	Cat#AM12230
Falcon 5 mL polystyrene round-bottom tube with cell strainer cap (Falcon 352235) (40 μm cell strainer)	Fisher Scientific	Cat#10585801
Microfuge tubes 1.5 mL	Thermo Fisher Scientific	Cat#AM12450
TPP TubeSpin bioreactor tubes	Merck	Cat#Z761028-180EA