Lysis Buffer

Reagent	Final concentration	Stock Concentration	Add to 25 mL
NaCl	35 mM	1 M	875 μL
Tris-HCl-pH 7.4	20 mM	1 M	500 μL
β-Mercaptoethanol	1 mM	13.5 M	2 μL
Superase inhibitor	1 U/mL	1,000 U/mL	25 μL
RNase-free DNase I	10 U/mL	10,000 U/mL	25 μL
EDTA-free protease inhibitor	N/A	N/A	1 tablet
ddH2O			23.5 mL
Total			25 mL