Extraction buffer 2 (make fresh)
| Reagent | Final concentration |
|---|---|
| Milli-Q ddH2O | n/a |
| HEPES pH 7.9 | 50 mM |
| NaCl | 150 mM |
| Sodium deoxycholate | 9.5% |
| EDTA | 1 mM |
| Protease inhibitor cocktail | 1 tablet for 10 mL extraction buffer |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.