| Reagents | Amount |
|---|---|
| Annealed oligos (from 100 μM stock) | 1.5 μL |
| Plasmid | 100 ng |
| T4 ligase buffer (10×) | 1 μL |
| T4 ligase (5 units/μL) | 0.5 μL |
| ddH2O | complete to 10 μL |
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