CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish

. 2020 Dec 10;1(3):100208. doi: 10.1016/j.xpro.2020.100208

Reagents	Final Concentration	Amount
Tryptone	40 mM	10 g
Yeast extract	15.6 mM	5 g
NaCl	170 mM	10 g
Agar (for solid medium only)	60 mM	20 g
ddH₂O	–	Adjust volume to 1 L

Aliquot 300 mL in 500 mL bottles.

Autoclave (121°C for 20 min) and store at 22°C–25°C for several months.

For preparing LB agar plates follow standard procedures. Briefly, after autoclave (or melting in a microwave) allow the solution to cool down ~50°C. Add antibiotic(s) and swirl to mix. Pour ~20 mL per 10 cm Petri dish. Plates can be stored at 4°C for 1 month.