FIG. 3.

Genotyping of several tissues from a live calf with high HDR frequencies. (A) Biopsies from several tissues in addition to ear and blood, including intestine, spleen, liver, lung, heart, pancreas, kidney, and tail were harvested and DNA isolated for genotyping. The PCR amplicons were purified, digested with AccI and resolved on agarose gel to identify successful gene targeting outcomes. (B) High throughput targeted amplicon sequencing on the iSeq platform. FastQ sequence output files were analyzed using the CRISPResso 2.0 platform. % of HDR, NHEJ, unmodified and other events (insertions, transpositions, other events) were binned and shown in the graph. Results from all tissues identified events within a smaller range, highlighting the homogeneity of editing across all tissues.