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. 2020 Dec 17;3(6):523–534. doi: 10.1089/crispr.2020.0047

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© Ki-Eun Park, et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — Genotyping of spermatozoa from a live calf with high HDR frequencies. (A) Genomic DNA from spermatozoa was isolated for genotyping. The PCR amplicons were purified, digested with AccI, and resolved on agarose gel to identify successful gene targeting outcomes. Genomic DNA from euthanized calf with high HDR edits (789 E) and wild type cells were used as controls. We could not recover semen from one of the electro-ejaculated bull calf (827) and was not incorporated in the iSeq run. (B) The % of HDR, NHEJ, unmodified and other events (insertions, transpositions, other events) were binned and shown in the graph.