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. Author manuscript; available in PMC: 2020 Dec 23.
Published in final edited form as: Biochemistry. 2020 Dec 4;59(49):4638–4645. doi: 10.1021/acs.biochem.0c00778

Figure 1.

Figure 1.

SARS-CoV-2 N1 TaqMan RT-qPCR assays performed using NEB Taq DNA polymerase and N gene armored RNA in indicated buffers. Buffer compositions are detailed in Table 2. Representative amplification curves resulting from duplicate analysis of 3 × 105 (black traces), 3 × 104 (red traces), 3 × 103 (blue traces), 3 × 102 (pink traces), 30 (green traces), and 0 (gray) copies of SARS-CoV-2 N gene armored RNA are depicted.