Table 1.
CDC TaqMan RT-qPCR primers and probes for SARS-CoV-2a (Adapted with permission from https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html Copyright (2020) Center for Disease Control and Prevention (CDC)).
| Name | Description | Sequenceb |
|---|---|---|
| 2019-nCoV_N1-F | 2019-nCoV_N1 Forward Primer | 5′-GACCCCAAAATCAGCGAAAT-3′ |
| 2019-nCoV_N1-R | 2019-nCoV_N1 Reverse Primer | 5′-TCTGGTTACTGCCAGTTGAATCTG-3′ |
| 2019-nCoV_N1-P | 2019-nCoV_N1 Probe | 5′-FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1–3′ |
| 2019-nCoV_N2-F | 2019-nCoV_N2 Forward Primer | 5′-TTACAAACATTGGCCGCAAA-3′ |
| 2019-nCoV_N2-R | 2019-nCoV_N2 Reverse Primer | 5′-GCGCGACATTCCGAAGAA-3′ |
| 2019-nCoV_N2-P | 2019-nCoV_N2 Probe | 5′-FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1–3′ |
| 2019-nCoV_N3-F | 2019-nCoV_N3 Forward Primer | 5′-GGGAGCCTTGAATACACCAAAA-3′ |
| 2019-nCoV_N3-R | 2019-nCoV_N3 Reverse Primer | 5′-TGTAGCACGATTGCAGCATTG-3′ |
| 2019-nCoV_N3-P | 2019-nCoV_N3 Probe | 5′-FAM-AYCACATTGGCACCCGCAATCCTG-BHQ1–3′ |
| RP-F | RNAse P Forward Primer | 5′-AGATTTGGACCTGCGAGCG-3′ |
| RP-R | RNAse P Reverse Primer | 5′-GAGCGGCTGTCTCCACAAGT-3′ |
| RP-P | RNAseP Probe | 5′-FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ-1–3′ |
a
According to CDC, oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves. Refer to CDC website for latest updates.
b
FAM: 6-carboxyfluorescein; BHQ-1: Black Hole Quencher 1