Table 3.

Average Ct values of Taq DNA polymerase-mediated TaqMan RT-qPCR assays of RNA templates.

SARS-CoV-2 genomic RNA in Gen 6 A buffer
Template copies/reaction	NEB Taq			Thermo Fisher Taq
	N1 assay	N2 assay	N3 assay	N1 assay	N2 assay	N3 assay
6000	26.81 ± 0.93	25.64 ± 1.42	26.76 ± 1.27	32.02 ± 1.25	38.52 ± 0.34	33.28 ± 4.62
600	29.96 ± 0.64	28.81 ± 0.97	30.12 ± 0.89	35.81 ± 1.26	41.28 ± 1.47	36.54 ± 5.02
60	33.33 ± 0.45	32.24 ± 0.87	33.10 ± 0.64			38.31 ± 1.00
6	37.14 ± 0.91	35.62 ± 1.45	36.90 ± 1.10
Nunber of replicates	6	6	6	3	3	3
Armored SARS-CoV-2 N RNA in Gen 6 A buffer				Armored RNaseP RNA in Gen 6 A buffer
Template copies/reaction	NEB Taq			Template copies/reaction	NEB Taq	Thermo Fisher Taq
	N1 assay	N2 assay	N3 assay		RNase P assay	RNase P assay
300000	24.32 ± 1.04	21.30 ± 0.48	22.66 ± 0.56	300000	21.17 ± 0.06	30.42 ± 0.14
30000	27.25 ± 0.28	24.55 ± 0.37	25.79 ± 0.31	30000	24.53 ± 0.05	34.94 ± 0.13
3000	30.33 ± 0.36	27.93 ± 0.46	28.92 ± 0.72	3000	27.88 ± 0.19	37.95 ± 0.22
300	33.82 ± 0.43	31.39 ± 0.59	32.41 ± 0.23	300	31.11 ± 0.04
30	37.50 ± 0.60	34.59 ± 0.42	35.90 ± 1.21
Number of replicates	4	4	4	Number of replicates	3	3