Table 11.

WTA index PCR program

Step	Temperature	Time	Cycles
Hot start	95°C	3 min	1
Denaturation	95°C	30 s	8–9 (for less than 2 nM use 9 cycles)
Annealing	60°C	30 s
Extension	72°C	30 s
Final extension	72°C	1 min	1
Hold	4°C	∞	1