Table 17.
AbSeq/sample tag PCR 1 program
| Step | Temperature | Time | Cycles |
|---|---|---|---|
| Hot start | 95°C | 3 min | 1 |
| Denaturation | 95°C | 30 s | 11–15 (for more than 10K cells use 11cycles) |
| Annealing | 60°C | 3 min | |
| Extension | 72°C | 1 min | |
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | ∞ | 1 |
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