Table 18.
Pipetting scheme for flow cytometry antibody staining
| Antibody (clone) | Dilution (2× master mix) | μL to add for each sample |
|---|---|---|
| HLA-DR BV421 (L243) | 1:50 | 2 |
| CD4 BV510 (OKT4) | 1:50 | 2 |
| CD16 BV605 (3G8) | 1:50 | 2 |
| CD45 BV711 (HI30) | 1:50 | 2 |
| CD8 BV785 (SK1) | 1:50 | 2 |
| CD66b FITC (G10F5) | 1:50 | 2 |
| CD14 PerCp-Cy5.5 (MϕP9) | 1:50 | 2 |
| CD56 PE (MY31) | 1:12.5 | 8 |
| CD3 PE/Dazzle (UCHT1) | 1:50 | 2 |
| CD11c PE/Cy5 (B-ly6) | 1:25 | 4 |
| Siglec8 PE/Cy7 (7C9) | 1:50 | 2 |
| CD203c APC (NP4D6) | 1:50 | 2 |
| CD1c AlexaFluor700 (L161) | 1:50 | 2 |
| CD19 APC/Fire 750 (HIB19) | 1:25 | 4 |
| LIVE/DEAD fixable yellow dead cell stain | 1:50 (1:10 pre-dilution in PBS) | 2 |
| BD Horizon brilliant stain buffer | 1:5 | 20 |
| FcR blocking reagent, human | 1:50 | 2 |
| PBS | 38 | |
| Total | 100 |