Figure 4.

Effect of TSA, and 5-Aza on miR-148a and miR-152 expression. A2780CP cells were treated with 0.5 µM TSA for 48 h and 5 µM 5-Aza for 48 h and 72 h separately and expression of A) miR-148a and; B) miR-152 was assessed by Real time PCR.Combination effect of TSA and 5-Aza on expression of C) miR-148a and; D) miR-152 was measured after cells were treated with a combination of 0.5 µM TSA for 48 h and 5 µM 5-Aza for 48 h and 72 h. RNU6B was considered as the internal control. Values represent mean±SD, for four independent experiments, ***P ≤ 0.001