Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Drosophila melanogaster)	Pngl	GenBank	ID:35527 Dmel_CG7865
Gene (Drosophila melanogaster)	Ncc69	GenBank	ID: 39410 Dmel_CG4357
Gene (Mus musculus)	NGLY1	GenBank	ID: 59007
Gene (Mus musculus)	NKCC1	GenBank	ID: 20496	Slc12a2
Genetic reagent (Drosophila melanogaster)	Pngl-RNAi	Bloomington Drosophila Stock Center	RRID:BDSC_54853	y1 v1; P{y+t7.7 v+t1.8=TRiP.HMJ21590}attP40
Genetic reagent (Drosophila melanogaster)	Tubulin-GAL4	Bloomington Drosophila Stock Center	RRID:BDSC_5138	y1 w*; P{w+mC = tubP-GAL4}LL7/TM3, Sb1 Ser1
Genetic reagent (Drosophila melanogaster)	Tubulin-GAL80	Bloomington Drosophila Stock Center	RRID:BDSC_5190	y1 w[*]; P{w[+mC]=tubP-GAL80}LL9 P{w[+mW.hs]=FRT(w[hs])}2A/TM3, Sb1
Genetic reagent (Drosophila melanogaster)	Drosophila Genetics Reference Panel	Bloomington Drosophila Stock Center	Set of 194 strains, example strain: BDSC:55014, RRID:BDSC_55014
Genetic reagent (Drosophila melanogaster)	Pdf-GAL4	Bloomington Drosophila Stock Center	RRID:BDSC_6899	P{w[+mC]=Pdf-GAL4.P2.4}X, y1 w[*]
Genetic reagent (Drosophila melanogaster)	UAS-Pngl-RNAi	Bloomington Drosophila Stock Center	RRID:BDSC_42592	y1 sc* v1 sev21; P{y+t7.7 v+t1.8=TRiP.HMS02424}attP40
Genetic reagent (Drosophila melanogaster)	UAS-Ncc69-RNAi	Bloomington Drosophila Stock Center	RRID:BDSC_28682	y1 v1; P{y+t7.7 v+t1.8=TRiP.JF03097}attP2
Cell line (Mus musculus)	Mouse embryonic fibroblasts (MEF)	Jackson Labs	Primary line from mouse #027060,https://www.jax.org/strain/027060
Antibody	Anti-NKCC1 (Rabbit polyclonal)	Cell Signaling	Cat#14581, RRID:AB_2798524	IB: 1:1000
Antibody	Anti-TCF11/NRF1 (Rabbit monoclonal)	Cell Signaling	Cat#8052, RRID:AB_11178947	IB: 1:1000
Antibody	IRDye 800CW Goat-anti-rabbit	Abcam	Cat#216773	IB: 1:10,000
Commercial assay or kit	Cell Fractionation Kit	Cell Signaling	Cat#9038
Chemical compound, drug	Bortezomib	EMD Millipore	Cat# 179324-69-7
Software, algorithm	Genome Wide Association	Chow et al., 2016
Software, algorithm	Gene Set Enrichment Analysis	Subramanian et al., 2005
Software, algorithm	Evolution Rate Covariation	Clark et al., 2012
Software, algorithm	R	https://www.r-project.org/