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. 2020 Dec 23;9:e59545. doi: 10.7554/eLife.59545

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© 2020, Uchigashima et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — The effects of OE of Nlgn3Δ isoform or enhanced green fluorescent protein (EGFP; control) in hippocampal CA1 pyramidal neurons were compared between inhibitory inputs mediated by Pv+ (A–D) and Sst+ (E–H) inhibitory interneurons. (A and E) Sample traces of unitary inhibitory postsynaptic currents (uIPSCs). Left, superimposed averaged sample uIPSC traces (Untrans: black; trans: dark gray) induced by an action potential (AP). Middle and right, superimposed averaged sample traces of uIPSCs evoked by single (dark gray) and double (black) APs in Pv+ and Sst+ interneurons. uIPSCs are normalized to the first amplitude. The amplitude (B and F) and paired-pulse ratio (PPR) (C and G) of uIPSCs were plotted for each pair of transfected (Trans) and neighboring untransfected (Untrans) cells (open symbols). Bar graphs indicate mean ± SEM. (D and H) Synaptic connectivity between presynaptic inhibitory interneuron and postsynaptic untransfected (open bars) or transfected (black) pyramidal neurons. Numbers of cell pairs: Nlgn3Δ or EGFP at Pv+ synapse (33 pairs/19 mice and 27/20) and Sst+ synapses (20/11 and 26/15). The number of tested slice cultures is the same as that of cell pairs. n.s., not significant. Mann–Whitney U-test.

Figure 3—figure supplement 1. — The effects of OE of Nlgn3Δ isoform or enhanced green fluorescent protein (EGFP; control) in hippocampal CA1 pyramidal neurons were compared between inhibitory inputs mediated by Pv+ (A–D) and Sst+ (E–H) inhibitory interneurons. (A and E) Sample traces of unitary inhibitory postsynaptic currents (uIPSCs). Left, superimposed averaged sample uIPSC traces (Untrans: black; trans: dark gray) induced by an action potential (AP). Middle and right, superimposed averaged sample traces of uIPSCs evoked by single (dark gray) and double (black) APs in Pv+ and Sst+ interneurons. uIPSCs are normalized to the first amplitude. The amplitude (B and F) and paired-pulse ratio (PPR) (C and G) of uIPSCs were plotted for each pair of transfected (Trans) and neighboring untransfected (Untrans) cells (open symbols). Bar graphs indicate mean ± SEM. (D and H) Synaptic connectivity between presynaptic inhibitory interneuron and postsynaptic untransfected (open bars) or transfected (black) pyramidal neurons. Numbers of cell pairs: Nlgn3Δ or EGFP at Pv+ synapse (33 pairs/19 mice and 27/20) and Sst+ synapses (20/11 and 26/15). The number of tested slice cultures is the same as that of cell pairs. n.s., not significant. Mann–Whitney U-test.