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. 2020 Dec 23;9:e59545. doi: 10.7554/eLife.59545

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© 2020, Uchigashima et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — The effects of shNlgn3 or shCntl expression in hippocampal CA1 pyramidal neurons were compared between three different inhibitory inputs mediated by VGT3+ (A–D), Pv+ (E–H), and Sst+ (I–L) inhibitory interneurons. (A, E, and I) Sample traces of unitary inhibitory postsynaptic currents (uIPSCs). Left, superimposed averaged sample traces of uIPSC (Untrans: black; trans: dark gray) induced by an action potential (AP). Middle and right, superimposed averaged sample traces of uIPSCs evoked by single (dark gray) and double (black) APs in VGT3+ (A), Pv+ (E), and Sst+ (I) interneurons. uIPSCs are normalized to the first amplitude. The amplitude (B, F, and J) and paired-pulse ratio (PPR) (C, G, and K) of uIPSCs were plotted for each pair of transfected (Trans) and neighboring untransfected (Untrans) cells (open symbols). Bar graphs indicate mean ± SEM. (D, H, and L) Synaptic connectivity between presynaptic inhibitory interneuron and postsynaptic untransfected (open bars) or transfected (black) pyramidal neurons. Numbers of cell pairs: shNlgn3 or shCntl at VGT3+ synapses (28 pairs/15 mice and 37/17), at Pv+ synapse (12/4 and 16/5), and Sst+ synapses (26/11 and 21/10). The number of tested slice cultures is the same as that of cell pairs. n.s., not significant. Mann–Whitney U-test.

Figure 4—figure supplement 1. — The effects of shNlgn3 or shCntl expression in hippocampal CA1 pyramidal neurons were compared between three different inhibitory inputs mediated by VGT3+ (A–D), Pv+ (E–H), and Sst+ (I–L) inhibitory interneurons. (A, E, and I) Sample traces of unitary inhibitory postsynaptic currents (uIPSCs). Left, superimposed averaged sample traces of uIPSC (Untrans: black; trans: dark gray) induced by an action potential (AP). Middle and right, superimposed averaged sample traces of uIPSCs evoked by single (dark gray) and double (black) APs in VGT3+ (A), Pv+ (E), and Sst+ (I) interneurons. uIPSCs are normalized to the first amplitude. The amplitude (B, F, and J) and paired-pulse ratio (PPR) (C, G, and K) of uIPSCs were plotted for each pair of transfected (Trans) and neighboring untransfected (Untrans) cells (open symbols). Bar graphs indicate mean ± SEM. (D, H, and L) Synaptic connectivity between presynaptic inhibitory interneuron and postsynaptic untransfected (open bars) or transfected (black) pyramidal neurons. Numbers of cell pairs: shNlgn3 or shCntl at VGT3+ synapses (28 pairs/15 mice and 37/17), at Pv+ synapse (12/4 and 16/5), and Sst+ synapses (26/11 and 21/10). The number of tested slice cultures is the same as that of cell pairs. n.s., not significant. Mann–Whitney U-test.