Figure 6. Expression of Nrxn isoforms in different types of inhibitory interneurons in the hippocampal CA1 region.

Double FISH for αNrxn1 (A, B, and C), αNrxn3 (D, E, and F), and βNrxn3 (G, H, and I) mRNAs in VGT3+ (A, D, and G), Pv+ (B, E, and H), and Sst+ (C, F, and I) in the hippocampus showing different levels of Nrxn mRNA (green) expression in different inhibitory interneurons (magenta). Note that the signal intensity in individual GABAergic neurons is variable, compared with that in glutamatergic neurons. Nuclei were stained with DAPI (blue). Or, st. oriens; Py, st. pyramidale; Ra, st. radiatum. (J) Summary scatterplots for αNrxn1, αNrxn3, and βNrxn3 mRNAs in VGT3+, Pv+, and Sst+ inhibitory interneurons. Data are represented as mean ± SEM. ns, not significant; ***p<0.001 (Mann–Whitney U-test). Scale bars, 20 μm.

Figure 6—figure supplement 1. Expression of Nrxn isoforms in different types of inhibitory interneurons in the hippocampal CA1 region.

(A–F) Single FISH for αNrxn1 (A), αNrxn2 (B), αNrxn3 (C), βNrxn1 (D), βNrxn2 (E), and βNrxn3 (F) in the hippocampus. Striking signals for αNrxn1 (A), αNrxn3 (C), and βNrxn3 (F) are noted in sparse cells in the st. radiatum (arrows). (G–O) Double FISH for βNrxn1 (G, H, and I), αNrxn2 (J, K, and L), and βNrxn2 (M, N, and O) in VGT3+ (G, J, and M), Pv+ (H, K, and N), and Sst+ (I, L, and O) in the hippocampus showing different levels of Nrxn mRNA (green) expression in different inhibitory interneurons (magenta, arrows). Note that the signal intensity in individual GABAergic neurons is variable, compared with that in glutamatergic neurons. Nuclei were stained with DAPI (blue). Or, st. oriens; Py, st. pyramidale; Ra, st. radiatum. Note that none of the Nrxn isoforms displayed unique expression specific to VGT3+ inhibitory interneurons (see Figure 6J). (P) Summary scatterplots for βNrxn1, αNrxn2, and βNrxn2 mRNAs in VGT3+, Pv+, and Sst+ inhibitory interneurons. Data are represented as mean ± SEM. ns, not significant; *p<0.05. Scale bars, 200 µm (A–F) and 20 μm (G–O).