Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 23;9:e59545. doi: 10.7554/eLife.59545

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020, Uchigashima et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 8. — (A–C) Effect of pre- and postsynaptic overexpression of αNrxn1 and Nlgn3Δ, respectively, on unitary inhibitory synaptic transmission in organotypic slice cultures prepared from VGT3/NrxnTKO/RFP mice. (A) Configuration of triple whole-cell recording (left top), superimposed fluorescent, and Nomarski images (right top). (Bottom) Individual fluorescence and Nomarski images of TdTomato (left) and tag-blue fluorescent protein (tag-BFP; right). (B) Averaged sample unitary inhibitory postsynaptic current (uIPSC) traces. Nrxns and Nlgn3Δ were transfected in TdTomato-positive and CA1 pyramidal neurons, respectively, by electroporation. (C, D, and E) Summary of uIPSC amplitude (C), paired-pulse ratio (D), and connectivity (E). Numbers of cell pairs: αNrxn1+AS4, αNrxn1-AS4, and BFP at VGT3+ synapses (seven pairs/three mice, 9/4 and 9/4). The number of tested slice cultures is the same as that of cell pairs. Mann–Whitney U-test.