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. 2020 Dec 14;9:e55730. doi: 10.7554/eLife.55730

Figure 6. Open chromatin and expression of CMF genes in ECs requires in vivo cues.

Figure 6.

(A) Experimental scheme. N = 2–4 biological replicates. ATAC-qPCR and ATAC-Seq was performed on freshly isolated endothelial nuclei, or cultured endothelial cells from heart or lung. qPCR regions were identified based on peaks identified in sequencing data. (B) Relative accessibility (compared to housekeeping genes, Rpsa and Tbp of endothelial cell genes, Cdh5 and Erg). (C) Relative accessibility of cardiomyocyte genes (Tnnt2, Myh6). (D) Relative accessibility of fibroblast-signature genes (Pdgfrb, Cspg4, Tcf21) (E) Representative TSS enrichment plot of cultured cardiac endothelial cells. Enrichment shown for cardiomyocyte-specific, endothelial or fibroblast specific gene sets (gene sets previously identified in Supplementary file 1). (F) GO enrichment for peaks that open (left) or close (right) in cultured cardiac endothelial cells vs freshly isolated. Complete statistical analyses by DESEQ2 and GO annotations shown in Supplementary file 4.