Figure 2.

Anesthesia and surgery elevate tau acetylation and decrease the neuronal SIRT1 expression in the hippocampus of aged rats. (a)–(d) Representative immunoblot bands and the corresponding densitometry analysis of ac-tau (k280), ac-tau (k686), SIRT1 expression normalized to β-actin, and p-tau (AT8) normalized to total tau. (e) Immunostaining of SIRT1 (red) and NeuN (green) in the control and sevoflurane+surgery groups. Data are presented as the mean ± SD. n = 6 per group. Scale bar = 50 μm.