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. 2020 Dec 14;2020:6685043. doi: 10.1155/2020/6685043

Figure 4.

Figure 4

Allicin treatment inhibited AOPP-induced oxidative stress and mitochondrial dysfunction of human NP cells. (a, b) The human NP cells were pretreated with different concentrations of allicin (0, 5, 10, and 20 μM) for 2 h, before treatment with 400 μg/ml AOPP for 24 h. The intracellular ROS levels of the NP cells for each group were detected by ROS-specific fluorescent probe DHE and measured by subsequent flow cytometry analysis. Representative peak charts of flow cytometry and relative quantitative analysis were shown. (c) The intracellular MDA levels (as a marker of lipid peroxidation) of human NP cells were examined by a commercial kit. (d, e) The mitochondrial membrane potential of human NP cells in each group was examined by JC-1 staining and measured by subsequent flow cytometry analysis. The quantitative analysis of the ratio of red fluorescence (y axis) to green fluorescence (x axis) and representative scatter plots of flow cytometry were shown. Data were represented as mean ± SD. #p < 0.05 and ##p < 0.01 versus the control group; ∗p < 0.05 and ∗∗p < 0.01 versus the AOPP alone treatment group, n = 3.