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. 2020 Dec 15;2020:8871745. doi: 10.1155/2020/8871745

Figure 1.

Figure 1

Effects of blue light irradiation on cell proliferation and TRPV1 expression. HaCaT cells were irradiated using blue light with a wavelength of 470–480 nm once daily for three consecutive days (a). On the fourth day, 24 h after the last irradiation, cell proliferation was measured using cell counting kit-8 assay (a) and EdU imaging analysis (b). The protein levels of TRPV1 were also determined by Western blotting analysis (c). HEK293-TRPV1 cells were irradiated using the indicated intensity of blue light with a wavelength of 470–480 nm (d). Twenty-four hours after the irradiation, the cells were harvested and subjected to luciferase reporter assay. Data are presented as the mean ± SEM of four independent experiments. Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey's multiple comparison test, using the GraphPad Prism 5 software. ∗p < 0.05 vs. the control group.