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ER stress induction with pharmacological inducer of ER stress, DTT, in EA.hy926 endothelial cells. Relative mRNA expression by RT-qPCR of ER stress markers: BiP (A), CHOP (B), GRP94 (C), and ATF-4 (D) normalized against housekeeping gene β-actin. Dithiothreitol (DTT) was used in concentration of 2 mM for 24 h to induce ER stress (n = 3–4). Data are presented as mean ± SEM. Data were analyzed by one-way ANOVA and Tukey's multiple comparison test. ***P < 0.001 vs. control (CTL).
