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. 2020 Dec 23;10:22375. doi: 10.1038/s41598-020-78758-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Application of the Antigen presentation (AP) based Monte Carlo epitope hotspot prediction method to mutated viral sequences from 10 different geographical locations, as well as the Wuhan reference sequence. Each group of bars shows the count of epitope hotspots found in the re- spective viral protein in each location. The epitope hotspot counts are shown for three different bin lengths: 27 (left), 50 (centre) and 100 (right). It is clear that the epitope hotspots are robust across mutation sequences. Only those hotposts that were identified for AP in Fig. 3 are illustrated in the plot.