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. 2020 Dec 23;10:22375. doi: 10.1038/s41598-020-78758-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The scatter plots show the distribution of the hotspot conservation scores (Y-axis) for proteins in the viral genome (X-axis). Each plot con- tains the conservation scores of the hotspots identified based on immune presentation (IP), using different bin sizes: 27 (left), 50 (center), and 100 (right). A hotspot is represented by a filled coloured circle, while the median conservation score for a given protein is depicted by a hollow square. As a reference, upwards facing triangles show the minimum conservation score for that protein. Only hotspots with a conservation score above the median were taken for further considertion.