TABLE 2.

Comparison between miRNAs datasets.

13 common elements	Missiaglia et al., 2017 “RMS vs. sk ms” Sample type: Tissue				Tombolan et al., 2020 “RMS vs. HD” Sample type: Plasma
ID	logFC	AveExpr	P-Value	FDR	FC	log FC	P-value
hsa-let-7d-5p	−0,32	4,37	0,00621581	0,02569494	−5,34	−2,42	0,00088406
hsa-miR-181b-5p	0,39	4,11	0,0019584	0,01013688	−2,10	−1,07	0,01209047
hsa-miR-191-5p	−0,31	3,54	0,00732697	0,0291309	−3,81	−1,93	0,00937036
hsa-miR-19a-3p	1,01	3,72	2,5271E-09	3,7729E-07	2,34	1,23	0,0003896
hsa-miR-19b-3p	0,48	3,98	0,0002534	0,00200527	1,74	0,80	0,00608738
hsa-miR-206	−0,50	4,62	0,00054228	0,00362842	108,93	6,77	0,00051132
hsa-miR-23a-3p	−0,45	4,13	0,00029463	0,00228758	−1,91	−0,93	0,00460076
hsa-miR-23b-3p	−0,84	3,79	2,0847E-07	7,7988E-06	−1,91	−0,93	0,01025567
hsa-miR-26a-5p	−0,48	4,29	6,2407E-05	0,00068482	−4,49	−2,17	0,00399244
hsa-miR-29a-3p	−1,27	3,34	9,9963E-08	4,33E-06	2,06	1,04	0,0072167
hsa-miR-29c-3p	−1,38	3,17	8,7252E-13	7,1808E-10	2,39	1,26	0,00454803
hsa-miR-30b-5p	−0,61	3,67	4,8404E-05	0,00055329	−3,66	−1,87	0,00268519
hsa-miR-30c-5p	−0,71	3,78	1,2507E-05	0,00018715	−5,91	−2,56	3,8173E-05

Matching of circulating miRNAs differently expressed in RMS and HD (right panel) plasma samples (Tombolan et al., right) and in RMS tumors and skeletal muscle cells (Missiaglia et al., left). Almost 50% (13 out of 30) of circulating miRNAs identified by the qPCR Cancer Panel assay were also found deregulated in RMS tumors. miR26a-5p and miR30b/30c showed comparable fold change values in the two datasets, whereas MiR-206, miR-29a/c, which are muscle-specific miRNAs, are deregulated in skeletal muscle cells (Missiaglia dataset) but up-regulated in RMS patients’ plasma compared to healthy donor’s plasma samples (qPCR Cancer Panel). In bold miRNAs with a concordant expression. logFC: log2 of the fold change; AveExp: average log2-expression; FDR: false discovery rate.