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. 2020 Dec 10;8:596788. doi: 10.3389/fcell.2020.596788

FIGURE 4.

FIGURE 4

The effects of DHA on activation of Shh pathway and stemness in ESCC cells. (A) The structure of DHA. (B) The effect of DHA at 2 μM (low concentration, DHA-L) and 20 μM (high concentration, DHA-H) on expression of Gli1 in the nucleus and cytoplasm in TE-1 cells. Histone3 and β-actin expression were used as nuclear and total loading controls, respectively. (C) The effect of DHA on the expressions of PTCH and MDR1 at mRNA level in TE-1 cells. *P < 0.05, compared with DMSO treated group cells. (D) Effect of DHA on the viability of TE-1 cells with or without Shh overexpression vector transfection. The cells were transfected with Shh overexpression or mock vector (control) for 48 h, and then treated with different concentrations of DHA for another 48 h. (E) The effects of DHA on the expression of Sox2 and Oct4 proteins in TE-1 cells. β-actin expression was used as a loading control. (F) The effect of DHA on the proportion of ALDH1 positive cells in TE-1 cells. All error bars are s.e.m. *P < 0.05, compared with control group.