FIGURE 5.

Starch amounts at end of day and end of night (A) and degradation of starch (B) in Nicotiana tabacum expressing mislocalized plastid phosphoglucoisomerase (pPGI). Starch in plants with mislocalized pPGI was significantly higher than empty vector control plants. Starch breakdown in empty vector plants was linear through the night, however, starch breakdown was non-linear in mislocalized pPGI plants. Each bar or data point represents mean and error bars represent SE, (n = 5 biological replicates). Bars or data points with an asterisk (*) are significantly different from corresponding empty vector samples as determined by Student’s t-test (P < 0.05) (two tailed and assuming unequal variance). Significance for linearity was determined by Prob(F) of the linear regressions.