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. 2020 Dec 10;10:576362. doi: 10.3389/fonc.2020.576362

Figure 2.

Figure 2

Novel genes targeted and regulated by MeCP2 in breast cancer. (A) Pie graph showing enriched pathways of ChIP-hits for MDA-MB-468 and MCF7 cell lines generated by Reactome pathway analysis. (B) ChIP-Seq experiments for IgG and MeCP2 were performed in MDA-MB-468. ChIP followed by end-point PCR was performed to validate 60 genes targeted by MeCP2. (C) Representative of two-independent RT-qPCR-based analysis to evaluate expression changes of MeCP2, EGFR, IL6, NUPR2, PSPH, CDH1, MAP1B, METTL7A, MALAT1, NEAT1, AND SRA1 genes in MDA-MB-468 (NTC, sh1 MeCP2, and sh3 MeCP2) cells. Transcript levels were normalized to actin transcript levels.