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. 2020 Dec 10;10:576362. doi: 10.3389/fonc.2020.576362

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Copyright © 2020 Castro-Piedras, Vartak, Sharma, Pandey, Casas, Molehin, Rasha, Fokar, Nichols, Almodovar, Rahman and Pruitt

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acetylation on K135 lysine residue does not alter sub-cellular localization of MeCP2 but influences its binding at gene promoters in MDA-MB-468 cells. (A) Immunofluorescence staining of empty vector (EV), HA-tagged MeCP2 (WT), K135R mutant (HA-K135R), and K135Q mutant (HA-K135Q) cells. Merge of HA-MeCP2 (green) and nuclear staining (blue) proteins is shown as HA/DAPI for each of the cells. Merge of DAPI (blue), HA-MeCP2 (green), and actin (red) is shown as MERGE for each of the cells. (B) Immunofluorescence staining of EV, WT, HA-K135R, and K135Q cells at higher magnification.