Figure 1.

Endometrial cells secrete exosomes

(A) Whole cell lysates (WCLs) of Ishikawa and HEC-1-A cells were immunoblotted for the receptive markers MUC1 and GAPDH as a loading control. (B) DiI-labeled endometrial epithelial cells were coincubated with HTR8/SVneo cells in a Transwell plates (membrane pore = 0.3 μm). (C) Representative electron microscopic images Ishikawa and HEC-1-A cell lines. Scale bars, 500 nm. Red arrows in image indicate MVBs containing typical ILVs. (D) The number of MVBs per cell profile. (E) The number of ILVs per MVBs. The number of MVBs and ILVs was counted randomly, and only MVBs containing typical ILVs were counted. The results were plotted as dot plots (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001).