Figure 6.

sEV-derived miR-100-5p promotes angiogenesis

(A) Wounds created when HUVECs grew to the proper density. Then, HUVECs were incubated with sEVs derived from Ishikawa cells or transfected with miR-100-5p mimics and allowed to migrate for 24 h. Bar, 500 μm. (B and C) The relative area of the open wound was counted and plotted after treatment with sEV or miRNA mimics, respectively. (D) HUVECs were stimulated with Ishikawa-sEVs or miR-100-5p mimics for 24 h, and the proliferation of cells was examined using the EdU assay. Bar, 50 μm. (E and F) The proliferation rate of HUVECs in (D) was counted and plotted. (G) Representative pictures of tube formation were shown (treatment with PBS or Ishikawa-sEVs). Number of branches and total element lengths were quantified. Bar, 50 μm. (H) Tube-formation assay in HUVECs was performed after treatment with miR-100-5p mimics. The schematic diagram of representative tubes was obtained using Angiogenesis Analyzer for ImageJ. The angiogenesis was quantified and counted. Bar, 50 μm (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001).